Microbiology
Mohamed Mohamed Ali; Salwa Mahmoud Helmy; Hanan Ali Fahmy; Haitham Elaadli; Ibrahim Elsayed Eldesoukey
Articles in Press, Accepted Manuscript, Available Online from 26 April 2024
Abstract
Staphylococcus aureus (S. aureus) is one of the most common etiological agents of mastitis worldwide. The current study aimed to determine the prevalence and antimicrobial resistance patterns of S. aureus in mastitic milk samples collected from several camel farms located in Matrouh Governorate, Egypt. ...
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Staphylococcus aureus (S. aureus) is one of the most common etiological agents of mastitis worldwide. The current study aimed to determine the prevalence and antimicrobial resistance patterns of S. aureus in mastitic milk samples collected from several camel farms located in Matrouh Governorate, Egypt. In that context, a total of 200 mastitic camel milk samples were evaluated for S. aureus using a conventional culture-based method and a molecular-based approach. The antibiotic susceptibility testing of S. aureus isolates was investigated using the procedures of disc diffusion and agar dilution methods. The antibiotic resistance genes were identified by polymerase chain reaction using particular primers. Of the samples screened, 60 (30%) were verified for S. aureus. S. aureus isolates displayed the highest level of resistance against Piperacillin-tazobactam (55%) followed by Trimethoprim-sulphamethoxazole (45%) and Amoxycillin (40%). Fifty % of the isolates were multidrug-resistant. The antimicrobial resistant determinants such as methicillin-resistant gene (mecA), beta-lactamase gene (blaZ), tetracycline resistance gene (tetK), erythromycin resistance gene (ermB) and vancomycin resistant gene (vanA) were detected in percentage of 100%, 100%, 95%, 90% and 20% of the isolates, respectively. In conclusion, the occurrence of multiple drug resistant S. aureus as a causative agent of clinical camel mastitis represents a major veterinary issue and also a potential public health hazard. Furthermore, the obtained findings unequivocally demonstrate the necessity for appropriate use of antibiotics in camel farms in Egypt and further molecular methodologies to identify the complete genetic profile for the recovered antimicrobial resistant S. aureus isolates.
Doha Elsayed Naeim; Ibrahim Elsayed Eldesoukey; Amgad Ahmed Moawad; Ashraf Mohammed Ahmed
Volume 14, Issue 5 , May 2023, , Pages 243-248
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) infection is a major public health problem. Therefore, this study was aimed to estimate the prevalence of MRSA in various food products. A total number of 204 food samples including raw milk (n = 30), cheese (n = 60), chicken (n = 25), beef (n = 24) ...
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Methicillin-resistant Staphylococcus aureus (MRSA) infection is a major public health problem. Therefore, this study was aimed to estimate the prevalence of MRSA in various food products. A total number of 204 food samples including raw milk (n = 30), cheese (n = 60), chicken (n = 25), beef (n = 24) and fish (n = 65) were collected from August to November of 2021 within different localities in Kafr El-Sheikh governorate, the northern region of Egypt. All samples were assessed through a series of bacteriological and biochemical techniques to identify MRSA. Out of 204 samples, 52(25.49%) isolates were presumptively identified as MRSA on oxacillin resistance screening agar base media. Of these 52 isolates, 17(32.69%) were characterized as coagulase-positive. For the molecular confirmation of MRSA, all isolates were subjected to polymerase chain reaction assays to detect mecA and mecC. In addition, mecA was identified in all the isolates (100%), whereas, none was positive for mecC. Therefore, based on the detection of mecA, the overall occurrence rate of MRSA among the samples was 8.33%. The isolates were also subjected to antimicrobial susceptibility tests. Cefoxitin, cefuroxime, oxacillin and amoxicillin-clavulanic acid were completely resistant (100%) to the isolates, however, susceptible to vancomycin and ciprofloxacin. Raw milk had the highest prevalence of MRSA (13.30%), followed by chicken (12.00%), fish (9.20%), cheese (5.00%) and beef (4.20%). Due to the possibility of transmission of these strains to humans, the high prevalence of MRSA in various foodstuffs in Egypt poses a potential public health risk.
